What is the tissue culture room? Tissue culture laboratory detailed introduction
The tissue culture room is also called the tissue culture laboratory, which is a professional basic laboratory for biotechnology and biological sciences. 1. The composition of the tissue training room: Â Â Â The tissue culture room is mainly composed of three parts: preparation room, inoculation room and culture room. 2. The main equipment of the tissue training room: Â Â Â Plant tissue culture is carried out under strict aseptic conditions, so it needs to be equipped with ultra-clean workbench, electronic analytical balance, precision pipette, autoclave, stereo microscope, light incubator, biochemical incubator, air conditioner, Refrigerators and other equipment. 3. Tissue training room construction The ideal tissue culture laboratory should be established in a quiet, clean, and far away from the source of pollution, preferably in the upwind direction of the main wind direction throughout the year to minimize pollution. It is best to build a tissue culture laboratory for large-scale production in a place with convenient transportation to facilitate the transportation of culture products.
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1. Preparation room: carry out all the preparation work related to the experiment, complete the preparation, weighing, dissolution, washing of the used mother liquor, preparation and distribution of medium, sterilization of medium and culture vessels, and pretreatment of culture materials Wait.
2. Inoculation room: used for the disinfection of plant materials, inoculation, transfer of cultures, subculture of test tube seedlings, preparation of protoplasts, and all technical procedures that require aseptic operations.
3. Cultivation room: a place where the in vitro plant material inoculated into the culture bottle is cultivated under controlled conditions.
The construction of a laboratory needs to consider two issues: one is the nature of the experiment being undertaken, that is, whether it is productive or research-based, whether it is basic or higher; the second is the scale and scale of the laboratory Mainly depends on the funding and the nature of the experiment.
Regardless of the nature and scale of the laboratory, the basic principles of laboratory setting are: scientific, efficient, economical and practical. A tissue culture laboratory must meet three basic needs: experimental preparation (medium preparation, utensil washing, medium and culture utensil sterilization), aseptic operation and controlled culture. In addition, the auxiliary laboratory and its various additional facilities can also be considered according to the experimental requirements to make the laboratory more complete.
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